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Publications

 

Hilbert, D. W., W. L. Smith, S. G. Chadwick, G. Toner, E. Mordechai, M. E. Adelson, J. D. Sobel, and S. E. Gygax. (2016) Development and validation of a highly accurate quantitative real-time PCR assay for the diagnosis of bacterial vaginosis. J Clin Microbiol

Schuyler JA, Mordechai E, Adelson ME, Sobel JD, Gygax SE, Hilbert DW. (2015) Identification of intrinsically metronidazole-resistant clades of Gardnerella vaginalis. Diagn Microbiol Infect Dis. pii: S0732-8893(15)00360-0. doi: 10.1016/j.diagmicrobio.2015.10.006. [Epub ahead of print]

Schuyler JA, Chadwick SG, Mordechai E, Adelson ME, Gygax SE, Hilbert DW. (2015) Draft Genome Sequence of a Metronidazole-Resistant Gardnerella vaginalis Isolate. Genome Announc. 3(5). pii: e00992-15. doi: 10.1128/genomeA.00992-15.

Schuyler, J. A., E. Mordechai, M. E. Adelson, S. E. Gygax, and D. W. Hilbert. (2015) Draft Genome Sequence of a Metronidazole-Resistant Derivative of Gardnerella vaginalis Strain ATCC 14019. Genome Announc

Schuyler JA, Mordechai E, Adelson ME, Gygax SE, Hilbert DW. (2015) Draft Genome Sequence of a Metronidazole-Susceptible Atopobium vaginae Isolate. Genome Announc. 3(5). pii: e00991-15. doi: 10.1128/genomeA.00991-15.

Smith WL, Hedges S, Mordechai E, Adelson ME, Trama J, Gygax SE, Kaunitz AK, Hilbert DW. Cervical and vaginal flora is highly concordant with respect to bacterial vaginosis-associated organisms and commensal lactobacillus species in reproductive-aged women. J. Clin. Micrbiol. In Press.

Whaley S, Caudle K, Vermitsky J-P, Chadwick SG, Toner G, Barker K, Gygax SE, Rogers PD. UPC2A is required for high-level azole antifungal resistance in Candida glabrata. Antimicrob. Agents Chemother. In Press.

Paulish-Miller TE, Augostini P, Schuyler JA, Smith WL, Mordechai E, Adelson ME, Gygax SE, Hilbert DW. (2014) Trichomonas vaginalis Metronidazole Resistance is Associated with Single Nucleotide Polymorphisms in the Nitroreductase Genes ntr4Tv and ntr6Tv. Antimicrob. Agents Chemother. 58(5): 2938-2943.

Balashov SV, Mordechai E, Adelson ME, Sobel JD, Gygax SE. (2014) Multiplex qPCR assay for the identification of major vaginal lactobacilli. Diagn. Microbiol. Infect. Dis. 78(4): 321-327.

Balashov SV, Mordechai E, Adelson ME, Gygax SE. (2014) Identification, Quantitation, and Subtyping of Gardnerella vaginalis in Noncultured Clinical Vaginal Samples by qPCR. J. Med. Microbiol. 63(2): 162-175.

Gallo-Ebert C, Donigan M, Stroke I, Swanson R, Manners M, Francisco J, Toner G, Gallagher D, Huang C-Y, Gygax S, Webb M, Nickels J. 2013. Novel antifungal drug discovery based on targeting pathways regulating the fungal-conserved Upc2 transcription factor. Antimicrob Agents Chemother [ePub ahead of print].

Steier Z, Vermitsky JP, Toner G, Gygax SE, Edlind T, Katiyar S. 2013. Flucytosine antagonism of azole activity versus Candida glabrata: Role of transcription factor Pdr1 and multidrug transporter Cdr1. Antimicrob Agents Chemother 57(11):5543-5547.

Chadwick SG, Prasad A, Smith WL, Mordechai E, Adelson ME, Gygax SE. 2013. Detection of epidemic USA300 community-associated MRSA strains using a single allele-specific PCR targeting a novel polymorphisms of Staphylococcus aureus pbp3. J Clin Microbiol 51(8):2541-2550.

Sobel JD, Subramanian C, Foxman B, Fairfax M, Gygax SE. 2013. Mixed vaginitis-more than coinfection and with therapeutic implications. Curr Infect Dis Rep 15(2):104-108.

Chadwick SG, Schuyler JA, Vermitsky JP, Adelson ME, Mordechai E, Gygax SE. 2013. X-Plate Technology™: a new method for detecting fluconazole resistance in Candida species. J Med Microbiol 62:720-726.

Balashov S, Mordechai E, Adelson ME, Gygax SE. 2012. Multiplex bead suspension array for screening Neisseria gonorrhoeae antibiotic resistance genetic determinants in noncultured clinical samples. J Mol Diagn 15(1):116-29.

Flowers SA, Barker KS, Berkow EL, Toner G, Chadwick SG, Gygax SE, Morschhäuser J, Rogers PD. 2012. Gain-of-Function mutations in UPC2 are a frequent cause of ERG11 upregulation in azole-resistant clinical isolates of Candida albicans. Eukaryot Cell 11(10):1289-1299.

Chadwick SG, Hart CE, Mordechai E, Adelson ME, Gygax SE. 2012. Staphylococcal Cassette Chromosome mec (SCCmec) type and antibiotic susceptibility profiles of vaginal and non-vaginal MRSA clinical isolates. Diagn Microbiol Infect Dis 74(2):210-212.

Hilbert DW, Paulish-Miller TE, Tan CK, Carey AJ, Ulett GC, Mordechai E, Adelson ME, Gygax SE, Trama JP. 2012. Clinical Escherichia coli isolates utilize alpha-hemolysin to inhibit in vitro epithelial cytokine production. Microbes Infect 14(7-8):628-638.

Gallo-Ebert C, McCourt PC, Donigan M, Villasmil ML, Chen W, Pandya D, Franco J, Romano D, Chadwick SG, Gygax SE, Nickels JT Jr. 2012. Arv1 lipid transporter function is conserved between pathogenic and nonpathogenic fungi. Fungal Genet Biol 49(2): 101-113.

Hilbert DW. 2011. Uropathogenic E. coli: the pre-eminent urinary tract infection pathogen. In MM Rogers and ND Peterson (Ed.) E. coli Infections: Causes, Treatment and Prevention (pp. 1-67). Nova Science Publishers, Hauppauge, NY. In press.

Hilbert DW, Paulish TE, Mordechai E, Adelson ME, Gygax SE, Trama JP. 2009. Antimicrobial non-susceptibility of cervico-vaginal and rectal Escherichia coli isolates is associated with phylogeny and plasmid carriage. Eur J Clin Microbiol Infect Dis 28(11):1399-1403.

Gygax SE, Vermitsky JP, Self MJ, Chadwick SG, Mordechai E, Adelson ME, Trama JP. 2008. Antifungal Resistance of Candida glabrata Vaginal Isolates and the Development of a qRT-PCR-based Azole Susceptibility Assay. Antimicrob Agents Chemother 52(9):3424-3426.

Vermitsky JP, Self MJ, Chadwick SG, Trama JP, Mordechai E, Adelson ME, Gygax SE. 2008. A Survey of Vaginal-flora Candida species Isolates from Women of Different Age Groups by Use of Species-Specific PCR Detection. J Clin Microbiol 46(4):1501-1503.

Hilbert DW, Paulish TE, Mordechai E, Adelson ME, Trama JP. 2008. O serogroup, phylogeny and virulence factors of cervicovaginal and rectal Escherichia coli isolates. Eur J Clin Microbiol Infect Dis 27:1265-8.

Hilbert DW, Pascal KE, Libby EK, Mordechai E, Adelson ME, Trama JP. 2008. Uropathogenic Escherichia coli dominantly suppress the innate immune response of bladder epithelial cells by a lipopolysaccharide- and Toll-like receptor 4-independent pathway. Microbes Infect 10:114-21.

Gygax SE, Schuyler JA, Trama JP, Mordechai E, Adelson ME. 2007. Detection of Erythromycin and Clindamycin Resistance Genes in Group B Streptococcal Clinical Isolates and Cervicovaginal-Rectal Swabs. Microb Drug Resist 13(2):119-123.

Gygax SE, Schuyler JA, Kimmel LE, Trama JP, Mordechai E, Adelson ME. 2006. Erythromycin and Clindamycin Resistance in Group B Streptococcal Clinical Islolates. Antimicrob Agents Chemother 50(5):1875-1877.

Grant Funding

NIH 1RO1AI07394-01A2 (2008 – 2012). Candida glabrata Pdr1: Master Regulator of Azole Resistance. Co-PIs: Thomas D. Edlind (Drexel University College of Medicine) and Scott E. Gygax (Medical Diagnostic Laboratories, L.L.C) subcontract.

ABSTRACTS AND PRESENTATIONS

Chadwick SG, Schuyler JA, Balashov S, Adelson ME, Mordechai E, Gygax SE. 2013. Disruption of Gardnerella vaginalis biofilms with clindamycin and metronidazole. 53rd Interscience Conference on Antimicrobial Agents and Chemotherapy, Denver CO. Poster Presentation.

Balashov S, Konduru L, Mordechai E, Adelson ME, Gygax SE. 2011. A Survey of Neisseria gonorrhoeae Mutations Conferring Resistance to Penicillin, Ciprofloxacin, Cefixime, Tetracycline, Azithromycin, and Spectinomycin. 51th Interscience Conference on Antimicrobial Agents and Chemotherapy, Chicago, IL. Poster presentation.

Hilbert DW, Smith WL, Kaunitz AM, Mordechai E, Adelson ME, Trama JP, Gygax SE. 2011. Analysis of host antimicrobial protein production during bacterial vaginosis. 111th American Society for Microbiology General Meeting, New Orleans, LA. Poster presentation.

Balashov S, Mordechai E, Gygax SE, Adelson ME. 2010. Development of a Diagnostic Test for Screening of Mutations in Neisseria gonorrhoeae Conferring Resistance to Penicillin, Ciprofloxacin, Cefixime, Tetracycline, Azithromycin, and Spectinomycin. Association for Molecular Pathology Conference, San Jose, CA. Poster presentation.

Chadwick SG, Overmyer CL, Mordechai E, Adelson ME, Gygax SE. 2010. Utilizing Single Nucleotide Polymorphisms in Staphylococcus aureus pbp3 for the Detection of Community Associated MRSA. 50th Interscience Conference on Antimicrobial Agents and Chemotherapy, Boston, MA. Poster presentation.

Vermitsky JP, Chadwick SG, Toner GJ, Mordechai E, Adelson ME, Gygax SE. 2010. CgUpc2 in the Master Regulator of Ergosterol Biosynthetic Genes in Candida glabrata. 11th Candida and Candidiasis Conference. Miami, Florida. Poster presentation.

Hilbert DW, Paulish TE, Mordechai E, Adelson ME, Trama JP. 2010. Escherichia coli alpha-hemolysin inhibits epithelial cytokine production. American Society for Microbiology 110th General Meeting, San Diego, CA. Poster presentation.

Edlind TD, Vermitsky JP, Gygax SE, Katiyar SK. 2010. Drug-target interaction mediates upregulation of multidrug transporter gene CDR1 in Candida glabrata. 11th Candida and Candidiasis Conference. Miami, Florida. Platform Presentation.

Knight SA, Vermitsky JP, Gygax SE, Edlind TD. 2010. Novel fluconazole resistance mechanism in Candida glabrata involving ERG upregulation. 11th Candida and Candidiasis Conference. Miami, Florida. Poster presentation.

Chadwick SG, Mordechai E, Adelson ME, Gygax SE. 2009. Susceptibility Profiles of Community-Associated MRSA Isolates from Cervicovaginal Swab Samples. 49th Interscience Conference on Antimicrobial Agents and Chemotherapy, San Francisco, CA. Poster presentation.

Edlind TD, Vermitsky JP, Gygax SE, Katiyar SK. 2009. Drug-Target Interaction Mediates Upregulation of Multidrug Transporter Gene CDR1 in Candida glabrata. 49th Interscience Conference on Antimicrobial Agents and Chemotherapy, San Francisco, CA. Platform Presentation.

Prasad A, Basu P, Mordechai E, Adelson ME, Gygax SE. 2009. PBP4 Polymorphisms Generate Penicillin Tolerance in Group B Streptococcus. 109th American Society for Microbiology General Meeting, Philadelphia, PA. Poster presentation.

Hilbert DW, Paulish TE, Mordechai E, Adelson ME, Trama JP. 2009. Uropathogenic Escherichia coli inhibits the activity of nuclear-localized NF-kB to obscure a Toll-like Receptor-independent Inflammatory stimulus. American Society for Microbiology 109th General Meeting. Philadelphia, PA. Poster presentation.

Vermitsky JP, Self MJ, Chadwick SG, Katiyar SK, Edlind TD, Gygax SE. 2009. cAMP-PKA kinase Tpk3 negatively regulates Candida glabrata Pdr1. 109th American Society for Microbiology General Meeting, Philadelphia, PA. Poster presentation.

Prasad A, Basu P, Mordechai E, Adelson ME, Gygax SE. 2008. Penicillin Tolerance in Group B. Streptococcus. 48th Interscience Conference on Antimicrobial Agents and Chemotherapy, Washington D.C. Poster presentation.

Vermitsky JP, Katiyar S, Gygax SE, Edlind TD. 2008. Multidrug resistance transcription factor Pdr1 is activated by MAP kinase Slt2 and repressed by protein kinase A Tpk3 in the opportunistic yeast Candida glabrata. 2008 Yeast Genetics and Molecular Biology Meeting, Toronto, Canada. Poster presentation.

Hilbert DW, Paulish TE, Libby EK, Pascal KE, Mordechai E, Adelson ME, Gygax SE, Trama JP. 2008. O Serotype, Virulence Factors, Plasmid Replicons, and Antibiotic Resistance of Rectal and Cervico-vaginal Escherichia coli Isolates. 108th American Society for Microbiology General Meeting, Boston, MA. Poster presentation.

Vermitsky JP, Self MJ, Katiyar S, Mordechai E, Adelson ME, Edlind TD, Gygax SE. 2008. The cAMP Protein Kinase A signal transduction pathway negatively regulates Pdr1, the master regulator of azole resistance in Candida glabrata. 10th Candida and Candidiasis Conference. Jersey City, New Jersey. Poster presentation.

Katiyar S, Vermitsky JP, Gygax SE, Edlind TD. 2008. Anatomy of Candida glabrata Pdr1, master regulator of azole resistance: evidence for activation by MAP kinase Slt2. 10th Candida and Candidiasis Conference. Jersey City, New Jersey. Poster presentation.

Edlind TD, Vermitsky JP, Pfaller M, Gygax SE, Katiyar S. 2008. Molecular mechanisms behind flucytosine resistance and azole-flucytosine antagonism in Candida glabrata. 10th Candida and Candidiasis Conference. Jersey City, New Jersey. Poster presentation.

Gygax SE, Prasad A, Trama JP, Mordechai E, Adelson ME. 2007. Penicillin tolerance in Group B Streptococcus. 47th Interscience Conference on Antimicrobial Agents and Chemotherapy, Chicago, IL. Poster presentation.

Balashov SV, Mordechai E, Adelson ME. 2007. Development of a new high-throughput test for Cystic Fibrosis mutation screening. 13th Association for Molecular Pathology Annual Meeting. Los Angeles, California. Poster presentation.

Gygax SE, Schuyler JA, Trama JP, Adelson ME. 2007. A Novel ß-Hemolysin/Cytolysin Expression Phenotype in Group B Streptococcal Clinical Isolates. 107th American Society for Microbiology General Meeting, Toronto, Canada. Poster presentation.

Gygax SE, Prasad A, Schuyler JA, Trama JP, Mordechai E, Adelson ME. 2006. Penicillin tolerance in Group B Streptococcus. 46th Interscience Conference on Antimicrobial Agents and Chemotherapy, San Francisco, CA. Poster presentation.

Trama JP, Libby EK, Gygax SE, Mordechai E, Adelson ME. 2006. Differential induction of pro-inflammatory cytokines by vaginal and cervical epithelial cells in response to Group B Streptococci. The American Association of Immunologists. Boston, MA. Poster presentation.

Trama JP, Libby EK, Gygax SE, Mordechai E, Adelson ME. 2006. Host, pathogen, and drug interaction of an in vitro model of vaginal candidiasis. 9th Candida and Candidiasis Conference. Denver, CO. Poster presentation.

Gygax SE, Schuyler JA, Trama JP, Mordechai E, Adelson ME. 2005. Erythromycin and clindamycin resistance in Group B Streptococcus clinical islolates. 45th Interscience Conference on Antimicrobial Agents and Chemotherapy, Washington, DC. Poster presentation.

Latest News

10/2015 - FEMERIS publication: Schuyler JA, Mordechai E, Adelson ME, Sobel JD, Gygax SE, Hilbert DW. Identification of intrinsically metronidazole-resistant clades of Gardnerella vaginalis. in Diagn Microbiol Infect Dis..

9/2015 - FEMERIS publication: Schuyler JA, Chadwick SG, Mordechai E, Adelson ME, Gygax SE, Hilbert DW. Draft Genome Sequence of a Metronidazole-Resistant Gardnerella vaginalis Isolate. in Genome Announc..

9/2015 - FEMERIS publication: Schuyler JA, Mordechai E, Adelson ME, Gygax SE, Hilbert DW. Draft Genome Sequence of a Metronidazole-Susceptible Atopobium vaginae Isolate. in Genome Announc..

7/2014 - Genesis Biotechnology Group (GBG) sponsored a two-day symposium on June 18th and 19th entitled “The Vaginal Microbiome and Bacterial Vaginosis.” Read Full Story

7/2014 - FEMERIS publication: Smith WL, Hedges S, Mordechai E, Adelson ME, Trama J, Gygax SE, Kaunitz AK, Hilbert DW. Cervical and vaginal flora is highly concordant with respect to bacterial vaginosis-associated organisms and commensal lactobacillus species in reproductive-aged women. J. Clin. Micrbiol. In Press.

7/2014 - FEMERIS publication: Whaley S, Caudle K, Vermitsky J-P, Chadwick SG, Toner G, Barker K, Gygax SE, Rogers PD. UPC2A is required for high-level azole antifungal resistance in Candida glabrata. Antimicrob. Agents Chemother. In Press.

10/2013 - FEMERIS Research Associate, Geoffrey Toner, and Director Dr. Scott Gygax had a manuscript entitled, “Novel antifungal drug discovery based on targeting pathways regulating the fungal-conserved Upc2 transcription factor” accepted into the journal Antimicrobial Agents and Chemotherapy.

9/2013 - FEMERIS Director Dr. Scott Gygax presented work done by Research Associate Sean Chadwick in a poster entitled “Disruption of Gardnerella vaginalis biofilms with clindamycin and metronidazole” at the 53rd Interscience Conference on Antimicrobial Agents and Chemotherapy in Denver, Colorado.

8/2013 - FEMERIS Research Associate, Geoffrey Toner, and Director Dr. Scott Gygax had a manuscript entitled, “Flucytosine antagonism of azole activity versus Candida glabrata: role of transcription factor Pdr1 and multidrug transporter Cdr1” accepted into the journal Antimicrobial Agents and Chemotherapy.

5/2013 - FEMERIS Research Associates, Lamar Smith and Sean Chadwick, and Director Dr. Scott Gygax had a manuscript entitled, “Detection of epidemic USA300 community-associated MRSA strains using a single allele-specific PCR targeting a novel polymorphism of Staphylococcus aureus pbp3” accepted into the Journal of Clinical Microbiology.

1/2013 - FEMERIS Director Dr. Scott Gygax had a manuscript entitled, “Mixed vaginitis- more than coinfection and with therapeutic implications” accepted into the journal Current Infectious Disease Reports.

1/2013 - FEMERIS Research Associates, Jessica Schuyler and Sean Chadwick, and Director Dr. Scott Gygax had a manuscript entitled, “X-Plate Technology™: A new method for detecting fluconazole resistance in Candida species” accepted into the Journal of Medical Microbiology.